Bison/Buffalo BioPRYN Pregnancy Testing

BioPRYN® is a pregnancy detection tool for use in ruminant animals. It measures the presence of Pregnancy-Specific Protein B (PSPB) in the blood circulation of the animal. The PSPB is a protein that is only produced by the placenta of the growing fetus. Cows and heifers must be 40 days post-breeding and cows - 90 days post-calving. There is a chance for a false positive if the residual PSPB has not cleared the maternal system from cows that have calved more recently than 90 days ago.

How It Works

BioPRYN® begins with collection of a blood sample from the animal. The samples are then shipped to a laboratory where an enzyme-linked immunosorbent assay (ELISA) is performed. Reports may be downloaded directly into dairy management software or sent via email or fax.

Using BioPRYN assures accuracy. The test is 99 percent accurate in identifying open females, giving producers confidence to administer a prostaglandin shot and restart a synchronization program. There is as much as a 5 percent false-pregnancy rate from the test, primarily due to early embryonic death which is a challenge regardless of which method is used for pregnancy detection..

The Benefits: Why should bison producers use BioPRYN?

  • Allows the veterinarian to concentrate on preventative medicine and treatment
  • More cost-effective than rectal palpation
  • BioPRYN is convenient: Tests conducted according to the dairy manager’s schedule
  • Safer for embryos. With palpation methods, commonly 2 to 11 percent of embryos die.
  • More accurate than palpation. Inaccurate pregnancy calculations lead to unintended abortions when abortion-causing drugs are administered to a pregnant animal.
  • For recipients of an embryo transplant, we recommend collecting blood at 45 days of embryo age rather than 40 days.


Lab Test Method:  ELISA test for detection of PSPB in serum

Lab Test Time:  30-35 hours